Site-specific recognition of the bacteriophage mu ends by the mu a protein
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چکیده
منابع مشابه
Replacement of the bacteriophage Mu strong gyrase site and effect on Mu DNA replication.
The bacteriophage Mu strong gyrase site (SGS) is required for efficient replicative transposition and functions by promoting the synapsis of prophage termini. To look for other sites which could substitute for the SGS in promoting Mu replication, we have replaced the SGS in the middle of the Mu genome with fragments of DNA from various sources. A central fragment from the transposing virus D108...
متن کاملCloning of the A gene of bacteriophage Mu and purification of its product, the Mu transposase.
The bacteriophage Mu transposase (the Mu A gene product), which is absolutely required for both integration of Mu and replicative transposition during the lytic cycle, has been overproduced by cloning the gene on a plasmid under the control of the phage lambda PL promoter. The protein has been purified to near homogeneity from the lysate of heat-induced cells of a strain carrying the plasmid. T...
متن کاملBacteriophage Mu targets the trinucleotide sequence CGG.
Target specificity for bacteriophage Mu was studied using a new phage derivative that enabled cloning of Mu-host junctions from phage DNA. Insertions distributed throughout the chromosome showed no orientation bias with respect to transcription or replication polarity. Genes with a high frequency of the triplet CGG were preferred targets.
متن کاملTHE POMERON IN A MODIFIED MU LTIPERIPHERAL MODEL
There have been many attempts [I-31 to produce correct pomeron by putting the nparticle production amplitude expht, in the unitarity integral equation. It was found that for reasonable values of h one could not get the right picture of the slope of the pomeron as a function of energy S4 .W e show that theinterference termsin the unitary equation does not improve the slope either. However, a...
متن کاملIntroduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu.
The host range of coliphage Mu was greatly expanded to various genera of gram-negative bacteria by using the hybrid plasmic RP4::Mu cts, which is temperature sensitive and which confers resistance to ampicillin, kanamycin, and tetracycline. These drug resistance genes were transferred from Escherichia coli to members of the general Klebsiella, Enterobacter, Citrobacter, Salmonella, Proteus, Erw...
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ژورنال
عنوان ژورنال: Cell
سال: 1984
ISSN: 0092-8674
DOI: 10.1016/0092-8674(84)90017-5